Bioengineering Living Biohybrid Therapeutics for Synergistic H2S Gaseous-Photothermal Cancer Eradication

Abstract

Hydrogen sulfide-mediated gaseous therapies offer high efficacy and biosafety in cancer treatment, but conventional delivery strategies suffer from poor tumor specificity and uncontrollable release. This work develops Bac@CuS living biohybrids that integrate engineered Vibrio natriegens for in situ H2S production with self-mineralized copper sulfide nanoparticles. The platform combines H2S gaseous therapy and photothermal therapy, impairing mitochondrial function, inducing cancer-cell apoptosis, and achieving strong tumor inhibition in vivo.